Rapid Quantification of the Latent Reservoir for HIV-1 Using a Viral Outgrowth Assay
Figure 1
Two-step bead depletion procedure yields highly purified resting CD4+ T cells from HIV-1 infected patients.
A two-step negative selection strategy to purify resting CD4+ T cells from patient PBMC. (A) Representative FSC/SSC plot indicating live cell population after the two-step bead depletion procedure. (B) Representative dot plot indicating purity of resting CD4+ T cells. Purified cells were stained with antibodies to CD4 and HLA-DR.