Rhesus Monkey Rhadinovirus Uses Eph Family Receptors for Entry into B Cells and Endothelial Cells but Not Fibroblasts
Figure 6
Inhibition of KSHV cell-cell transmission into a B cell line by soluble Eph proteins and soluble Ephrins.
BJAB B cells were co-cultured for 10 days (A) or 4 days (B) with lytically induced iSLK.219 cells in the presence of the indicated proteins at 5 µg/ml. The cells were then harvested and analyzed for expression of the GFP reporter gene by flow cytometry. BJAB cells were distinguished from iSLK.219 cells by a two-step gating strategy, first selecting by FSC/SSC for the B cell population and then by gating for CD20 expression. The percentage of green CD20-positive cells from co-culture with non-induced iSLK cells represents the assay background. PBS and EGFR-Fc serve as controls. (n = 3; error bars indicate sd).