IL-27 Receptor Signalling Restricts the Formation of Pathogenic, Terminally Differentiated Th1 Cells during Malaria Infection by Repressing IL-12 Dependent Signals
Figure 7
The Foxp3+ Treg response is unaltered in WSX-1−/− mice during malaria infection.
WT and WSX-1−/− mice were infected with P. berghei NK65. (A) Representative plots showing the expression of Foxp3 vs Tbet by splenic CD4+ T cells from naive and infected WT and WSX-1−/− mice. (B–E) The (B, D) frequencies and (C, E) total numbers of splenic CD4+ T cells from naive and infected WT and WSX-1−/− mice expressing FoxP3 and splenic CD4+FoxP3+ T cells from naive and infected WT and WSX-1−/− mice expressing T-bet, respectively. (F) Representative plots showing the expression of Foxp3 vs IFN-γ by splenic CD4+ T cells from naive and infected WT and WSX-1−/− mice following in vitro stimulation with PMA and ionomycin. (G–H) The (G) frequencies and (H) total numbers of splenic CD4+FoxP3+ T cells from naive and infected WT and WSX-1−/− mice expressing IFN-γ. (I–J) The (I) frequencies and (J) total numbers of splenic CD4+FoxP3+ T cells from naive and infected (D14) WT and WSX-1−/− mice expressing CXCR3. The results are the mean +/− SEM of the group with 3–5 mice per group. The results are representative of 3 independent experiments. * P<0.05 between WT and WSX-1−/− mice.