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Genome-wide Prediction and Functional Validation of Promoter Motifs Regulating Gene Expression in Spore and Infection Stages of Phytophthora infestans

Figure 6

Interspecific conservation of motifs.

(A) Pie chart illustrating the fraction of the 103 motifs that were detected at the same (i.e. expected) location in alignments of orthologous promoters from P. infestans (Pi), P. ramorum (Pr) and P. sojae (Ps), in the same location in P. ramorum or P. sojae compared to P. infestans, in a new location in P. ramorum or P. sojae, or not detected outside of P. infestans. (B) Representative CLUSTAL alignments. Shown on the left side of the figure are alignments of PITG_16321 (containing M51) with its orthologs from P. ramorum and P. sojae. Conserved blocks to the left and right of M51 are labeled as LB and RB, respectively. Shown in the right column are alignments of PITG_03886, PITG_09960, and PITG_14222, which contain M58. Also marked is the Initiator core promoter element, INR, which normally spans the transcriptional start site.

Figure 6

doi: https://doi.org/10.1371/journal.ppat.1003182.g006