Dissection of Ire1 Functions Reveals Stress Response Mechanisms Uniquely Evolved in Candida glabrata
Figure 3
Growth assay in the presence of ER stress-inducing agents.
Logarithmic-phase cells of S. cerevisiae and C. glabrata strains were adjusted to 2×107 cells/ml, and then 5 µl of serial 10-fold dilutions were spotted onto synthetic complete (SC) plates containing either tunicamycin (TM) or dithiothreitol (DTT) at the indicated concentrations. Plates containing TM and DTT were incubated at 30°C for 24 and 48 h, respectively. S. cerevisiae strains: WT, BY4742; Δire1, BY4742Δire1; and Δhac1, BY4742Δhac1. C. glabrata strains: WT, CBS138; Δire1, TG121; and Δhac1, TG141.