Skip to main content
Advertisement

< Back to Article

Atomic Model of Rabbit Hemorrhagic Disease Virus by Cryo-Electron Microscopy and Crystallography

Figure 1

Electron microscopy and 3D image reconstruction of RHDV.

(A) Micrograph of purified, negatively stained RHDV (bar = 100 nm). (B) CryoEM micrograph of purified RHDV (bar = 40 nm). The black arrows and stars point to RHDV particles with differing amounts of internal density (also see Figure S1). (C) Reconstructed cryoEM map of the RHDV virion, color-coded by radius. Icosahedral 2-, 3- and 5-fold axes are indicated by black symbols and AB and CC capsomers are identified. On the right, the closest half of the density map has been removed to reveal internal features in the RHDV density map. The contour threshold of the cryoEM map here was set to 3.3σ above the mean. (D) Same as (C) but with the capsomer density removed to show just the RHDV inner shell. Insert: A region of the density map is shown as a transparent grey isosurface into which is fitted the backbone structures of the S domains from three copies of the RHDV major capsid protein, VP60 (A in red, B in yellow and C in blue). Magenta arrows point to a few representative density features that represent residue side chains.

Figure 1

doi: https://doi.org/10.1371/journal.ppat.1003132.g001