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Bifurcation of Arabidopsis NLR Immune Signaling via Ca2+-Dependent Protein Kinases

Figure 3

Synergism of CPKs and WRKYs on WRKY46 promoter activity.

(A) Requirement of W-boxes for WRKY46 promoter activity in protoplasts. The WT or mutant WRKY46 promoter was co-transfected with avrRpt2 or a vector control. The scheme represents the positions of four W-boxes in the WRKY46 promoter. (B) Functional genomic screen of WRKYs in protoplasts. The representative WRKY from different groups were co-transfected with CPKac5 for the activation of WRKY46 promoter. The bottom panel shows the expression of individual HA epitope-tagged WRKYs detected by Western blot. (C) Synergistic activation of WRKY46 promoter by WRKY48 and specific CPKacs in protoplasts. (D) Synergistic activation of WRKY46 promoter by WRKY28 and specific CPKacs in protoplasts. “m” indicates the kinase-dead mutants of CPKacs. (E) Synergistic activation of WRKY46 promoter by WRKY8 and specific CPKacs in protoplasts. “m” indicates the kinase-dead mutants of CPKacs. The above experiments were repeated three times with similar results.

Figure 3

doi: https://doi.org/10.1371/journal.ppat.1003127.g003