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Lymphocytic Choriomeningitis Virus Infection in FVB Mouse Produces Hemorrhagic Disease

Figure 3

FVB mice develop an acute lethal disease after LCMV-13 infection independent of reduced viral titers in tissues.

FVB or C57BL/6 mice were infected with 1–2×106 p.f.u. and monitored for up to 11-days post-infection for development of disease signs or death. Animals were humanely killed upon development of severe disease. A) Survival curves of FVB and C57BL/6 mice (n = 11). B) Survival curves of FVB mice infected with LCMV-13 and LCMV-Arm (n = 3 mice) C) Weight change of FVB (n = 8) and C57BL/6 (n = 10) mice starting from pre-infection levels. Results are presented as a mean percentage of initial weight +/− standard deviation. D) Viral RNA in tissues of FVB (n = 13) and C57BL/6 (n = 15) mice infected with LCMV-13. Levels of viral RNA in indicated tissues at time of death were quantified by qRT-PCR for LCMV RNA. Results are presented as mean +/− standard deviation (*, p<0.05. One-way Anova). E) Viral burden as assessed by plaque assay. Infectious virus was quantified day 7 post-infection for all mice (n = 5). Results are presented as mean +/− standard deviation (*, p<0.05. t-test). F) FVB mice treated with anti-arenavirus antisense did not exhibit increased survival to LCMV infection compared to PBS treated animals (n = 5).

Figure 3

doi: https://doi.org/10.1371/journal.ppat.1003073.g003