RelAp43, a Member of the NF-κB Family Involved in Innate Immune Response against Lyssavirus Infection
Figure 6
RelAp43 is involved in IFN-β transcription during lyssavirus infection.
The results presented are the means of five (A) or three (B, C, D) independent experiments. Error bars indicate standard deviations. *p<0.05. (A) Luciferase assay of IFN-β promoter activation in presence of M protein. M-Tha (black bars), M-SAD (dark grey bars), or CAT (light grey bars) as a control were used. The measure obtained with M-SAD was arbitrary set to 1. The expression levels of M-Tha, M-SAD and CAT in each condition were assessed by western blot (bottom of the figure). (B) Infectious titres of Tha and SAD viruses from supernatant of Hela cells infected at an MOI of 1 at 24 and 48 hours p. i. in the presence of siRNA control (siRNAc) or anti-RelAp43 siRNA (siRelAp43). Titers are given in Fluorescent focus units per ml (FFU/ml). (C) Decrease of the number of RelAp43 mRNA copies in Hela cells infected with Tha or SAD virus and in non-infected cells (NI) in the presence of anti-RelAp43 siRNA (siRNA RelAp43) compared to siRNA control (siRNAc) and measured by quantitative RT-PCR. The level of transcription of RelAp43 in Hela cells measured 48 hours after infection with SAD and in the presence of control siRNA was arbitrary set to 1. (D) Modulation of IFN-β mRNA levels detected by quantitative RT-PCR in HeLa cells transfected by either a control siRNA or an anti RelAp43 siRNA, and infected with Tha or SAD virus compared to non-infected cells (NI).