Hsp90 Inhibitors Are Efficacious against Kaposi Sarcoma by Enhancing the Degradation of the Essential Viral Gene LANA, of the Viral Co-Receptor EphA2 as well as Other Client Proteins
Figure 5
Central repeat domain of LANA resists degradation.
(A–C) HeLa cells were transfected with plasmids including full-length LANA, LANA mutant (deleted repeat central domain) and EBNA1 respectively in six-well plates, followed by treatment with 17-DMAG at concentrations of 0, 0.1, 0.5, 2.5 and 10 µM for 48 hours. Whole cells lysates of each sample were immunoblotted with anti-LANA and anti-Flag antibodies, Cdc2 and β-Actin were used as controls.