Binding of Glycoprotein Srr1 of Streptococcus agalactiae to Fibrinogen Promotes Attachment to Brain Endothelium and the Development of Meningitis
Figure 2
(A) GBS strain COH31 strain was incubated with wells pretreated with collagen, thrombin, fibrinogen, fibronectin, laminin, fetuin, or plasminogen (0.1 µM per well). Values represent mean ± S.D. of the percent of inoculum bound. (B) Immobilized fibrinogen was preincubated with rabbit anti-fibrinogen IgG (100 µg/ml) or rabbit IgG (100 µg/ml) prior to testing for binding by GBS. Unbound IgG was removed by washing and GBS binding was assessed. Values represent percent of GBS binding as compared with untreated fibrinogen. (C) Far Western blotting of cell wall proteins from strain COH1 (WT) and PS954 (Δsrr1). Left panel shows SDS-PAGE of cell wall preparations. Proteins were transferred to nitrocellulose and probed with purified fibrinogen (fibrinogen; 10 µg/ml, middle panel) or biotinylated WGA lectin (0.2 µg/ml, right panel). Bound fibrinogen and lectin were detected with anti-fibrinogen IgG or HRP conjugated streptavidin, respectively. * = P<0.01.