Opposing Roles for Interferon Regulatory Factor-3 (IRF-3) and Type I Interferon Signaling during Plague
Figure 4
IFNAR-dependent depletion of Ly6G+ cells in the bone marrow.
Bone marrow cells of wild type C57BL/6 and Ifnar−/− mice that were not infected (NI) (n = 3 mice per group assayed in 2 independent trials) or on day 5 post-challenge by intranasal infection with Y. pestis KIM D27 (n = 13 mice per group assayed in 3 independent trials) and stained with anti-Ly6G/6C-FITC followed by flow cytometry analysis. Data was gated on high stained cells as indicated in the scatter plot (A) and are shown for mice with the greatest percentage neutrophils in each group from a single trial. (B) Mean percent neutrophils from representative trial, error bars indicate the standard deviation from the mean. White bars indicate wild type mice, black bars indicate Ifnar−/− mice; *P<0.05 between wild type and Ifnar−/− mice determined by two-tailed Kruskal Wallis test.