HPV 5 and 8 E6 Abrogate ATR Activity Resulting in Increased Persistence of UVB Induced DNA Damage
Figure 4
HPV E6 expression increases sensitivity to crosslinking agents.
A. Median Lethal dose of UVB for HPV E6 expressing primary human foreskin keratinocytes (HFKs) was measured following exposure to a range of UVB doses (0–20 mJ/cm2). n≥5. Error bars represent standard errors of the mean. B. Median Lethal dose of UVB for HT1080 cells expressing the same HPV E6s was measured following a range of UVB doses (0–10 mJ/cm2). n≥5. Error bars represent standard errors of the mean. C. Relative viability of HPV E6 expressing HT1080 cells was measured after exposure to mitomycin C (µM). n≥5. Error bars represent standard errors of the mean. D. Relative viability was measured in HPV E6 expressing HT1080 cells following exposure to a range of cisplatin doses (µM). n≥5. Error bars represent standard errors of the mean. Linear regression lines were calculated using GraphPad Prism software. # denotes a statistical significant difference from LXSN for HPV 5, 38, and 16 E6 expressing cells (p≤0.05). * denotes a statistical significant difference from LXSN for all HPV E6 expressing cells (p≤0.05).