The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi
Figure 6
Cytokine production and immune modulators by “in vitro” T. cruzi infected DC, macrophages or cardiomyocytes.
Peritoneal macrophages, DC or cardiomyocytes from Slamf1−/− from BALB/c mice were infected in vitro with T. cruzi. A) Cox-2, iNOS and Arginase mRNA, evaluated by QC-PCR production (upper graphs) and protein by western blot (lower gels) by infected macrophages at 24 or 48 hr post infection as described in Methods. B) Cytokine (IFN-γ and TNF) release to supernatants from infected macrophages was evaluated by ELISA 24 or 48 hr post infection as described in Methods. C) Cytokine (IFN-γ and IL-12) release to supernatants from infected DCs was evaluated by ELISA 24 or 48 hr post infection as described in Methods. D) IFN-γ and NO production by infected cardiomyocytes. NO was evaluated by Gris reaction and IFN-γ by ELISA. Results are expressed as the mean values (±SD) for triplicates from 3 different experiments. (*) Statistically significant differences between Slamf1−/− and BALB/c cells (p>0.05).