Skip to main content
Advertisement

< Back to Article

Tomato TFT1 Is Required for PAMP-Triggered Immunity and Mutations that Prevent T3S Effector XopN from Binding to TFT1 Attenuate Xanthomonas Virulence

Figure 3

Reduced TFT1 mRNA expression in VIGS tomato leaves correlates with reduced PTI marker mRNA abundance in response to Xcv infection.

Relative mRNA levels for four PTI marker genes (PTI5, GRAS4, WRKY28, and LRR22) in 4 control (TRV2) and 4 TFT1-silenced (TRV2-TFT1) tomato lines. Leaflets on the same branch were inoculated with 1×105 CFU/mL of Xcv or Xcv ΔhrpF. Total RNA isolated from inoculated leaves at 6 HPI was used for Q-PCR. Actin mRNA expression was used to normalize the expression value in each sample. Error bars indicate SD for four plants. Asterisk indicates significant difference (t test, P<0.05) in the infected TRV2-TFT1 lines compared to the similarly infected TRV2 lines.

Figure 3

doi: https://doi.org/10.1371/journal.ppat.1002768.g003