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A Single Polar Residue and Distinct Membrane Topologies Impact the Function of the Infectious Bronchitis Coronavirus E Protein

Figure 8

Neither ssIBV E or FLAG-IBV E produce normal levels of VLPs.

(A) Immunoblot showing the amount of IBV N, M, and E in cells and released as VLPs. (10% of cell fraction, 100% of VLP fraction) (B) Quantification of immunoblot data showing the amount of M released with no E, IBV E, ssIBVE, or FLAG-IBV E. Data were normalized to the amount of M expressed in each sample, and the amount of M released with IBV E was set to 1 for ease of comparison. Data are from at least five independent experiments. Error bars represent +/− SEM, and the asterisk denotes a significant decrease in VLP level compared to IBV E by Student's t-test (p≤0.01).

Figure 8

doi: https://doi.org/10.1371/journal.ppat.1002674.g008