A Single Polar Residue and Distinct Membrane Topologies Impact the Function of the Infectious Bronchitis Coronavirus E Protein
Figure 8
Neither ssIBV E or FLAG-IBV E produce normal levels of VLPs.
(A) Immunoblot showing the amount of IBV N, M, and E in cells and released as VLPs. (10% of cell fraction, 100% of VLP fraction) (B) Quantification of immunoblot data showing the amount of M released with no E, IBV E, ssIBVE, or FLAG-IBV E. Data were normalized to the amount of M expressed in each sample, and the amount of M released with IBV E was set to 1 for ease of comparison. Data are from at least five independent experiments. Error bars represent +/− SEM, and the asterisk denotes a significant decrease in VLP level compared to IBV E by Student's t-test (p≤0.01).