A Single Nucleotide Polymorphism in Tetherin Promotes Retrovirus Restriction In Vivo
Figure 8
B6 Apobec3 is a dominant over NZW Tetherin restriction in vivo.
(A) Apobec3/Rfv3 genotype influence acute infectious viremia. Infectious viremia, as measured using the Mus dunni focal infectivity assay, was measured for the entire B1 cohort (n = 58). Apobec3/Rfv3r/s mice exhibited significantly lower infectious viremia. Apobec3/Rfv3r/s mice (n = 39) were genotyped as TetherinVal/Val versus TetherinMet/Val and various FV infection and pathogenesis parameters were compared. (B) Infectious viremia, split by Tetherin genotype from panel A. (C) Splenomegaly, based on wet spleen mass. (D) Bone marrow erythroblast percentages, quantified following FACS staining with Ter119 marker. In panels B to D, TetherinVal/Val and TetherinMet/Val exhibited similar levels of infectious viremia, spleen mass and bone marrow erythroblast percentages. Each dot corresponds to an infected mouse. Solid lines correspond to mean values and differences were evaluated using a 2-tailed Student's t test.