Essential Roles for Soluble Virion-Associated Heparan Sulfonated Proteoglycans and Growth Factors in Human Papillomavirus Infections
Figure 2
HPV16 and syndecan-1 release from the HaCaT cell plasma membrane is MMP dependent.
Immunoblot for syndecan-1 (snd-1) post starvation in SFM and after 6 h at 37°C in Tyrode's Buffer (A); immunoblot for HPV16 L1 released into CM post virus binding for indicated times (B) or 24 h (D). (C) Gelatin zymography showing protease activity present in HaCaT cell CM alone or with binding of HPV16. (D–F) Effect of MMP inhibitors batimastat (BM) or marimastat (MM) at the indicated concentrations on HPV16 release into media as in panel B and densitometric quantification with AlphaEaseFC software (E) and relative infection levels (F). Cells were untreated (U) or pre-treated with the indicated concentrations of BM, MM or TIMP3 for 1 h, then exposed to HPV16 PsV in the presence of inhibitors in CM. Panel D includes lanes spliced together from the same exposure of the same films. (F) Infection was assayed by quantifying luciferase levels at 24 h p.i. Data are represented as mean ± SEM of 3 experiments.