Systems Biology Approaches Reveal a Specific Interferon-Inducible Signature in HTLV-1 Associated Myelopathy
Figure 5
Cellular and viral responses to IFN in HTLV-1 carriers.
STAT1-P(Y701) phosphorylation in response to (A) IFN-α and (B) IFN-γ was quantified in whole blood by flow cytometry. Bar graphs represent the mean ± SEM. P-values were calculated using a two-tailed Mann-Whitney test with *P<0.05 versus controls, $P<0.05 versus AC; Ctrl: n = 6, AC: n = 6, HAM/TSP: n = 6. Effect of exogenous IFN-α on the expression of viral transcriptional transactivator (C) Tax (P = 0.4263), (D) the structural protein Gag (P = 0.0112) and (E) HBZ mRNA (P = 0.0742). Tax and Gag protein levels were assessed by flow cytometry, HBZ mRNA was quantified by real-time PCR. P-values were calculated using a paired Wilcoxon signed-rank test; Tax and Gag protein: HAM/TSP: n = 11; HBZ mRNA: AC: n = 2, HAM/TSP: n = 7.