A Host Small GTP-binding Protein ARL8 Plays Crucial Roles in Tobamovirus RNA Replication
Figure 5
Effects of TOM1 and ARL8 coexpression on the membrane-association of ToMV 130K protein.
The soluble (S15) and membrane (P15) fractions were prepared from yeasts expressing the indicated proteins and subjected to immunoblot analysis using antibodies against TOM1, ARL8, ToMV 130K, a yeast cytoplasmic protein Pgk1p, and an ER-localized protein Dpm1p. S15 samples prepared from 0.12 OD600 units of yeast cells or P15 samples prepared from 0.6 OD600 units of yeast cells were applied in each lane.