Down-Regulation of Shadoo in Prion Infections Traces a Pre-Clinical Event Inversely Related to PrPSc Accumulation
Figure 5
Sprn and Prnp gene expression time-course in mouse brain.
Mice infected with prions show no significant changes for Prnp and Sprn in three mouse group brains. (a) Prnpa allele-containing mice (FVB, C57Bl/6J) infected with RML or 301 V show almost no changes for Sprn and Prnp, while showing significant changes for GFAP gene transcripts (“Gfap”); (b) Inoculations into Prnpb mice, C57Bl/6.I-1, show similar trends for those genes with profiles in Prnpa allele mice; (c) other mouse strains with various PrPC concentration deriving from different gene copy-numbers infected with RML: FVB-Tg(PrP-A)4053 (Prnp over expressing), Prnp0/+ hemizygotes and Prnp0/0control mice that are resistant to prion infections. No significant changes were detected for Sprn and Prnp while significant changes were detected for GFAP only in Prnp0/+mouse brain. In each case the x-axis shows the incubation time post-inoculation in weeks and the y-axis shows the gene expression change as the log2 median ratio between prion-infected brain samples and control samples.