SAG101 Forms a Ternary Complex with EDS1 and PAD4 and Is Required for Resistance Signaling against Turnip Crinkle Virus
Figure 1
The EDS1-80 or EDS1-90 genes complement the compromised defense phenotypes in eds1-1 plants.
(A) Photograph showing phenotypes produced upon infiltration of 105 colony forming units (CFU)/ml of avrRps4 Pseudomonas syringae. The leaves were photographed at 6 days post inoculation (dpi). (B) Electrolyte leakage in avrRps4 inoculated genotypes. Error bars represent SD (n = 6). (C) PR-1 gene expression in avrRps4 inoculated plants. Leaves were sampled at 2 dpi. Ethidium bromide staining of rRNA was used as a loading control. (D) Salicylic acid (SA) and SA glucoside (SAG) levels in indicated genotypes at 0 and 48 h post inoculation with avrRps4. Asterisks indicate data statistically significant from wt Col-0 ecotype (P<0.05, n = 3). The error bars indicate SD. (E) Growth of avrRps4 bacteria on indicated genotypes. The error bars indicate SD. Asterisks indicate data statistically significant from wt (Col-0, P<0.05 n = 4). H and L indicate transgenic plants expressing high and low levels of EDS1, respectively (see Figures S4A, S4B).