Expression of the RAE-1 Family of Stimulatory NK-Cell Ligands Requires Activation of the PI3K Pathway during Viral Infection and Transformation
Figure 5
p110α PI3K is involved in the maintenance of RAE-1 and MULT-1 expression on transformed cells.
A) A20, NIH 3T3 and YAC-1 tumor cells were treated with 20 uM LY294002 for 24 hrs and stained for RAE-1 at the cell surface. Histograms show isotype control (shaded gray), untreated (solid black) and treated cells (dashed black). The histogram is a representative figure from multiple independent experiments. B) YAC-1 cells were treated with the indicated inhibitors at 10 uM, 5 uM, 2.5 uM, 1.25 uM, and 0.625 uM and surface stained for RAE-1. SD and statistical significance were determined from three independent experiments. Statistical significance (** or *) on the bottom corresponds to PI-103 and the ones on top correspond to rapamycin treatments. C) NIH 3T3 cells expressing RAE-1, MULT-1, and H60a were treated with the indicated inhibitors for 24 hrs and stained for RAE-1, MULT-1 or H60a (20 uM LY294002, 1 uM PI-103, 1 uM TGX-221, 100 nM Rapamycin, 10 uM AS252424, 10 uM IC87114, and 10 uM NU7026). Percent RAE-1 and ligand expression were determined by normalizing the MFI of RAE-1 or other ligands in inhibitor-treated cells to the MFI of RAE-1 or other ligands in DMSO-treated cells. SD and statistical analyses were calculated from three independent experiments. **p<0.01 and *p<0.05.