Viral Infection Induces Expression of Novel Phased MicroRNAs from Conserved Cellular MicroRNA Precursors
Figure 3
Phased miRNAs induced in RSV-infected rice.
(A–E) Precursors of phased miRNAs. The sequences shown in red and blue are reported miRNAs and miRNA*s, respectively. The histograms indicate the reads of corresponding sequences on the precursors shown in black bars. (F) RNA gel blot showing the expression of phased miRNAs in RSV-infected rice. Rice U6 was reprobed as a control. (G) Expression analysis of the predicted targets of phased miRNAs by real time RT-PCR. Os03g02240 was previously validated as a target of miR159.2. [61]. Os12g03530 and Os02g4700 are predicted targets of miR159a.3 and miR394.2, respectively. The expression levels of the assayed genes were normalized to the expression level of OsEF-1α.