In vivo Hypoxia and a Fungal Alcohol Dehydrogenase Influence the Pathogenesis of Invasive Pulmonary Aspergillosis
Figure 6
Normalized fold expression of the A. fumigatus alcC gene in vivo during fungal pathogenesis.
Outbred CD1 mice were immunosuppressed by single injection of Triamcinolone (40 mg/kg) 1 day prior to A. fumigatus intranasal inoculation. Lungs were harvested on indicated days and whole RNA was prepared. Quantification of mRNA was achieved by quantitative real-time PCR using fungal specific primers. Values are normalized to tefA and shown relative to mock control animals. Results are the mean and standard deviation of three replicates.