A Concerted Action of Hepatitis C Virus P7 and Nonstructural Protein 2 Regulates Core Localization at the Endoplasmic Reticulum and Virus Assembly
Figure 2
Characterization of JFH1 and Jc1 core localization by subcellular fractionation in HCVcc-expressing cells.
Untransfected (A) or Huh7.5 cells transfected with RNAs from the full-length genomes of JFH1 (B) and Jc1 (C) were lysed 72 h post-transfection and ca. 2 mg of protein lysates were fractionated on an iodixanol gradient. Each fraction was analyzed by Western blotting using antibodies against Calnexin, ADRP, HCV core and E2 proteins. 1/50 of the unfractionated post-nuclear extracts (PNE) were also analyzed. The infectious titers in each fraction were determined and illustrated as different categories with titers below to 1×103 FFU/fraction (white boxes), from 1 to 3×103 FFU/fraction (yellow boxes), from 3 to 6×103 FFU/fraction (orange boxes), and titers up to 6×103 FFU/fraction (red box).