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A Role for TLR4 in Clostridium difficile Infection and the Recognition of Surface Layer Proteins

Figure 1

Purification and characterisation of SLPs isolated from C. difficile.

Coomassie blue stained 12% SDS-PAGE gel showing crude extracts and purified C. difficile SLPs at each stage of the purification process. Individual high molecular weight (HMW) and low molecular weight (LMW) proteins and an irrelevant protein from the same process are also shown. Bone marrow derived DCs were isolated from Balb/C mice and incubated with either SLPs (20 µg/mL), LMW (20 µg/mL), HMW (20 µg/mL) or an irrelevant protein (20 µg/mL) for 24 h. LPS (100 ng/ml) was used as a positive control. The concentration of TNFα was measured in the supernatants by ELISA. The results are the mean (±SEM) for n = 4. *** p<0.001, determined by one-way ANOVA test comparing all groups.

Figure 1

doi: https://doi.org/10.1371/journal.ppat.1002076.g001