High Affinity Nanobodies against the Trypanosome brucei VSG Are Potent Trypanolytic Agents that Block Endocytosis
Figure 10
Alignment and functional characterization of Nb_An05 mutants.
(A) Alignment of the Nb_An05 mutant sequences with the wild type sequence; only the mutations in the complementarity determining regions are shown. (B) Flow cytometry analysis using wild type Nb_An05 and derived variants (Nb_An05-2,4,5,6,12,17,19), detected with mouse anti-6⋅His IgG and a phycoerythrin-labeled rat anti-mouse IgG (blue line). The black line represents controls with no Nb. For each experiment 2×105 parasites were incubated with 1 µg Nb (VSG∶Nb molar ratio of 1∶20).