MDA5 and TLR3 Initiate Pro-Inflammatory Signaling Pathways Leading to Rhinovirus-Induced Airways Inflammation and Hyperresponsiveness
Figure 3
RV1B-induced chemokine expression in MDA5−/− and TLR3−/− mice.
MDA5−/−, TLR3−/− and their strain control mice were inoculated with sham, UV-irradiated RV1B (UV RV1B) or intact RV1B. Lungs were harvested 24 h after infection. A–D. The expression of CXCL1/KC, CXCL2/MIP-2, CCL2/MCP-1, CXCL10/IP-10 and CCL11/eotaxin-1 was determined by qPCR. E–F. Protein production of CXCL1/KC and CXCL2/MIP-2 was measured by ELISA and bioplex assay. The expression of each target gene was normalized to GAPDH. Data represent mean±SEM for 3–7 mice (*p<0.05, one-way ANOVA).