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Acquisition of Human-Type Receptor Binding Specificity by New H5N1 Influenza Virus Sublineages during Their Emergence in Birds in Egypt

Figure 7

Growth kinetics of rEG/D1 viruses in avian cells and human cells.

(A) CEF cells were infected in triplicate with parental EG/D1 and five rEG/D1 viruses (rEG/D1, rEG/D1Q192H, rEG/D1129Δ,I151T, rEG/D1-EG/12 HA and rEG/D1-EG/12 HAH192Q) at an MOI of 0.1 or 0.01. (B) Human SAEC cells were infected in triplicate with the viruses at an MOI of 1 or 0.1. The culture supernatants were harvested at the indicated times and assayed for focus-forming units on CEF cells to determine the progeny virus titer (log10 FFU/ml). Each data point in (A) and (B) is the mean ± SD of triplicate experiments. (C) Phase contrast microscopy of morphological changes in SAEC cells infected by the indicated viruses at an MOI of 0.1 and examined at the indicated times post-infection.

Figure 7

doi: https://doi.org/10.1371/journal.ppat.1002068.g007