Human Cytomegalovirus IE1 Protein Elicits a Type II Interferon-Like Host Cell Response That Depends on Activated STAT1 but Not Interferon-γ
Figure 4
IE1 induces an IFN-γ-like transcriptional response.
TetR and TetR-IE1 cells were treated with doxycycline for 72 h and solvent (w/o), IFN-α, or IFN-γ for 24 h. Relative mRNA expression levels were determined by qRT-PCR with primers specific for a set of IE1-responsive genes (A), typical type I IFN response genes and IE1 itself (B). Results were normalized to TUBB, and mean values with standard deviations from two biological and two technical replicates are shown. ISG expression is shown in comparison to untreated TetR cells (set to 1). IE1 expression is presented relative to untreated TetR-IE1 cells (set to 1).