Chemokine Binding Protein M3 of Murine Gammaherpesvirus 68 Modulates the Host Response to Infection in a Natural Host
Figure 7
Array analysis of changes in pulmonary chemokine and cytokine levels within infected wood mice as a consequence of M3 loss.
Equal amounts of protein from lung lysates of wood mice infected with M3.stop or M3.MR MHV-68 were incubated with RayBiotech 3.1 membrane arrays capable of detecting 61 different chemokines and cytokines. Shown are the relative abundance of cytokines and chemokines (minus background) whose expression consistently showed more than a two-fold difference in M3.stop- versus M3.MR-infected mouse lung lysate (two independent experiments). Asterisks denote chemokines that have been tested in vitro and found to be bound by M3; # denotes chemokines tested and not bound by M3, according to van Berkel et al. [16] and Parry et al. [15]; + denotes that MIP-3/CCL19-dependent chemotaxis is inhibited by M3 according to Jensen et al. [46]; #* denotes that in the case of BLC/CXCL13, while Parry et al. [15] and Martin at al. [62] found that M3 bound weakly and inhibited factor-dependent chemotaxis, van Berkel et al. [16] did not observe any binding to M3.