Rapid End-Point Quantitation of Prion Seeding Activity with Sensitivity Comparable to Bioassays
Figure 3
Combined RT-QuIC end-point dilution analyses from four scrapie BH stocks.
Each 263K stock (Brain #1-#4 corresponding to Panels A-D) was serially diluted and used to seed RT-QuIC reactions. The percentage of replicate wells (n = 4–8) that are ThT-positive according to the criteria in Material and Methods was calculated and the mean percentage ± standard deviation from three separate experiments with each stock is shown. Animal bioassay data is represented as the percentage of replicate animals reaching the clinical stage of disease at each dilution of 263K scrapie BH (4–22 animals per dilution per stock).