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The Gag Cleavage Product, p12, is a Functional Constituent of the Murine Leukemia Virus Pre-Integration Complex

Figure 5

Analysis of the co-localization of p12 with MA or CA.

U/R cells were infected with the 1xMycR virus and analyzed by immunofluorescence, as described in Fig. 3. p12 (A–C), MA(A, B) and CA (C) were detected with the anti-Myc monoclonal antibody, goat anti-MA and goat anti-CA polyclonal antibodies, respectively, and the matched secondary FITC-conjugated donkey anti-mouse IgG and Red-X- conjugated donkey anti-goat IgG. For consistency, the images were pseudocolored to present p12 in red and MA or CA in green. Dashed lines mark the boundaries of cell-free regions where virions were detected attached to the cover glass. Bars represent 10 µm.

Figure 5

doi: https://doi.org/10.1371/journal.ppat.1001183.g005