Cryo Electron Tomography of Native HIV-1 Budding Sites
Figure 6
A previously undescribed Gag lattice in budding sites and released particles.
(A) Computational slice through cryo electron tomograms of U-373 MG cells transduced with AdGagPol. Budding sites (b) and released particles (p) exhibit a Gag lattice different from both the immature and the mature lattice. (B) Enlarged slice through the top budding site from (A). Density is observed at the expected position of the CA domain, but no prominent density corresponding to NC-RNA is detected. (C) For comparison, a slice through a budding site with the immature Gag lattice showing the characteristic two density layers underneath the plasma membrane. Thickness of computational slices through cryo electron tomograms is 1.6 nm. pm: plasma membrane. Scale bars are 100 nm.