Enterohemorrhagic E. coli Requires N-WASP for Efficient Type III Translocation but Not for EspFU-Mediated Actin Pedestal Formation
Figure 9
The WASP/N-WASP-binding region of EspFU is required for N-WASP-independent actin pedestal formation.
(A) Sequence alignment of an EspFU repeat and the corresponding VLL/AAA mutant. The WASP/N-WASP-binding α-helix is colored in blue. (B) NW−/− FLCs ectopically expressing HA-Tir-EspFU-2RWT or HA-Tir-EspFU-2RVLL/AAA fusions were challenged with an EPECΔtir strain (which expresses intimin), and stained with DAPI (blue), anti-HA antibody (green) and Alexa568-phalloidin (red).