Skip to main content
Advertisement

< Back to Article

Mouse Senile Amyloid Fibrils Deposited in Skeletal Muscle Exhibit Amyloidosis-Enhancing Activity

Figure 3

ApoA-II protein detected in amyloid fibril fractions isolated from various muscles.

A. ApoA-II protein in amyloid fibril fractions of the hindlimb muscles from six Apoa2cTg+/− mice injected with AApoAII (mouse-1 to mouse-6 in lane 1 to 6), and three R1.P1-Apoa2c mice without AApoAII injection (mouse-1 to mouse-3 in lane 7 to 9) were detected with anti-mouse apoA-II antiserum. 25 µg of protein were loaded into each lane. B. ApoA-II protein found in various muscles of Apoa2cTg+/− mouse-1 injected with AApoAII (Lane 1 to 4) and a non-injected R1.P1-Apoa2c mouse-1 (lane 5 to 8). Lane 1 and 5: forelimb triceps brachii muscles. Lane 2 and 6: hindlimb femoral quadriceps. Lane 3 and 7: longissimus thoracis muscles in the back. Lane 4 and 8: greater pectoral muscles from the breast. C. ApoA-II protein found in various muscles of R1.P1-Apoa2c mice (two or four months after injection with AApoAII fibrils). Lane 1, 5, 9, 13, 17 and 21; forelimb triceps brachii muscles. Lane 2, 6, 10, 14, 18 and 22: hindlimb femoral quadriceps muscles. Lane 3, 7, 11, 15, 19 and 23: longissimus thoracis muscles in the back. Lane 4, 8, 12, 16, 20 and 24: greater pectoral muscles.

Figure 3

doi: https://doi.org/10.1371/journal.ppat.1000914.g003