YfiBNR Mediates Cyclic di-GMP Dependent Small Colony Variant Formation and Persistence in Pseudomonas aeruginosa
Figure 5
Downstream components of the YfiBNR system.
A) Colony morphologies of mutants lacking potential downstream targets of the YfiBNR system grown on LB Congo Red agar. 1 = PA01, 2 = ΔyfiR, 3 = ΔyfiR pelG::Tn, 4 = ΔyfiR ΔpslAB, 5 = ΔyfiR ΔpslAB pelG::Tn, 6 = ΔyfiR cupA4::Tn, 7 = ΔyfiR cupB4::Tn, 8 = ΔyfiR cupC2::Tn. B) Attachment of mutants lacking potential downstream targets of the YfiBNR system, relative to PA01. C) YfiBNR effects on downstream gene transcription. Values are expressed as the percentage of luminescence reporter gene expression in ΔyfiR or ΔyfiBNR, as compared to PA01 wild type. The control strain contains pME6032-luxCDABE. Transcription of pel is massively up-regulated in ΔyfiR, and down regulated in ΔyfiBNR.