Degradation of Host Sphingomyelin Is Essential for Leishmania Virulence
Figure 9
Detection of neutral SMase activity in L. amazonensis promastigotes and axenic amastigotes.
Whole cell lysate from L. amazonensis promastigotes (pro) and axenic amastigotes (ama) were incubated with NBD-labeled C6 sphingomyelin and neutral SMase activity was determined by TLC (A) and normalized to nmol/µg/hour (B). Positive control (+): 0.1 unit of B. cereus SMase; negative control (−): boiled L. major WT lysate. Experiments were repeated multiple times and error bars represent standard deviations. *, p<0.05.