Dengue Virus Capsid Protein Usurps Lipid Droplets for Viral Particle Formation
Figure 5
Targeting the C protein to LDs is necessary for DENV production.
A. The media of BHK cells transfected with DENV RNA WT or mutants (Mut α1, Mut α1–α2 loop, Mut α2, Mut α2.1, and Mut α2.2) were collected as a function of time post-transfection and used to quantify the amount of infectious particles by plaque assay in BHK cells. The plot indicates the plaque forming units per ml at different times post-transfection. B. The secreted enveloped protein E was analyzed in the supernatant of transfected cells by western blot as previously described [33]. C. BHK cells were infected with a multiplicity of infection of 0.1 of WT, Mut α2.1, and Mut α2.2 viruses. The viral RNA was quantified by real time RT-PCR in the media obtained 24 h post-infection.