CD8+ T Cells Restrict Yersinia pseudotuberculosis Infection: Bypass of Anti-Phagocytosis by Targeting Antigen-Presenting Cells
Figure 4
CD8+ T cells are Required to Protect Against Y. pseudotuberculosis.
Naïve mice were given phosphate-buffered saline (PBS, black circles) or CD8-depleting antibody 2.43 (α-CD8, grey circles) prior and subsequent to intravenous inoculation with 102 CFU ksgA− bacteria, sacrificed at day 14 post-inoculation and CFU enumerated in the spleen (A) and liver (B). Open symbols indicate the limit of detection for that organ, each symbol represents one mouse, and lines are median values. Data shown is pooled from three separate experiments. (C) To examine protection in immune animals, at 60 days post-oral ksgA− immunization, mice received PBS (black circles) or CD8-depleting antibody 2.43 (α-CD8, grey circles) prior and subsequent to intravenous inoculation with 104 CFU virulent YPIII pIB1 (ksgA+) bacteria. Morbidity and mortality was followed for 14 days post-challenge and % survival shown by Kaplan-Meier plots. Data is representative of two experiments.