Two HIV-1 Variants Resistant to Small Molecule CCR5 Inhibitors Differ in How They Use CCR5 for Entry
Figure 10
Neutralization of parental and VVC-resistant clones by rabbit anti-V3 peptide sera.
Anti-peptide sera (day 49) from rabbits R1 (V3-CC1/85; closed squares), R2 (V3-CC1/85; closed circles), R3 (V3-CC101.19; open squares) and R4 (V3-CC101.19; open circles) were tested for their neutralizing activity against entry of Env-pseudotyped viruses (A) CC1/85 cl.7; (B) CC1/85 cl. 6; (C) CC101.19 cl.7; (D) D1/85 cl.23 into U87-CD4-CCR5 cells. The percent neutralization by immune sera was normalized to the effect of the pre-immune sera at the corresponding dilution. In most case, the effect of pre-immune sera on viral entry was negligible. The values shown are the means±SEM from two independent experiments.