Chemical Genetics Reveals Bacterial and Host Cell Functions Critical for Type IV Effector Translocation by Legionella pneumophila
Figure 9
Real time analysis of effector translocation by L. pneumophila.
A. Real time detection of CCF4 hydrolysis mediated by TEM-LepA translocation in J774 cells from L. pneumophila at various multiplicity of infection (MOI). B. Effect of MOI on the apparent maximal rate of product accumulation mediated by translocated TEM-LepA effector. The blue dotted line represents the ideal correlation between MOI and the apparent maximal rate of product accumulation (correlation coefficient of 1). C. Effect of antibody opsonization of the translocation efficiency of TEM-RalF, TEM-LepA and TEM-LegA3. D. Rate of product accumulation mediated by translocated TEM-RalF, TEM-LepA or TEM-LegA3 effectors as function of time in the presence or absence of antibodies. Legend of panel C applies also to panel D. Rates of product accumulation are expressed as arbitrary unit per unit of time (min.). The presented data are from a representative experiment.