Dual Role for Pilus in Adherence to Epithelial Cells and Biofilm Formation in Streptococcus agalactiae
Figure 2
Pilus synthesis and localization in S. agalactiae sortases mutants.
Proteins anchored to the cell-wall or associated to the membrane or secreted in the supernatant were isolated from S. agalactiae strain NEM316 and its isogenic sortases mutants [22], separated on 4%–12% Criterion XT SDS-PAGE gel, and detected by immunoblotting with specific anti PilB antiserum (upper part). Equivalent amount corresponding to 500 µl of the initial culture was loaded in each well. The PilB monomer is indicated by a black arrow. The high-molecular-weight species correspond to PilB polymers while the lower band at 50 kDa is most likely a degradation product. The equal quantity loaded in each well is verified by immunoblotting the same gel with a control antiserum that recognizes a secreted protein Bsp of 65 kDa (lower part).