Endothelial Targeting of Cowpea Mosaic Virus (CPMV) via Surface Vimentin
Figure 2
CPMV virions bind specifically to vimentin.
(A) VOPBA and (B) α-vimentin western blot of enriched plasma membrane isolates of the following samples 1 = HUVEC, 2 = HeLa, 3 = KB, 4 = MFT-6 vim+/+, 5 = MFT-16 vim−/−; 6 = purified CPMV particles, 7 = recombinant vimentin protein. (C) Proteins were immobilized on ELISA plate, incubated with CPMV in varying molar concentrations and probed for CPMV attachment through antibody detection. Immobilized proteins were vimentin (squares), BSA (triangles) and no protein (open circles). Bars represent mean+/−s.d. of triplicate samples.