A Novel Pathogenicity Gene Is Required in the Rice Blast Fungus to Suppress the Basal Defenses of the Host
Figure 4
The deletion of DES1 caused the induction of strong plant defense responses.
(A) DIC and fluorescence microscopy of infected rice sheaths (Nakdongbyeo) 48 h after inoculation. DIC images were captured using an 80-ms exposure time of transmission light with a DIC filter. Fluorescence images were captured using a 500-ms exposure for absorbed light using a GFP filter. Arrowheads on DAB staining panel indicate appressorium. Bar = 30 µm. (B) The expression of rice pathogenesis-related (PR) genes over time after inoculation. The transcriptional expression of PR1a and PBZ1 in the infected rice was analyzed using quantitative RT-PCR.