Deletions in the Repertoire of Pseudomonas syringae pv. tomato DC3000 Type III Secretion Effector Genes Reveal Functional Overlap among Effectors
Figure 5
HopR1 suppresses callose formation by the ΔIVΔCEL mutant in N. benthamiana.
N. benthamiana leaves were infiltrated with the ΔIVΔCEL mutant strains transformed with pBBR PavrPto avr/hop expression constructs at 5×108 CFU/ml with a blunt syringe. Aniline blue-stained callose depositions in N. benthamiana leaves were visualized by epifluorescence microscopy 12 h after inoculation. Numbers are the means and standard deviations of eight 1-cm2 microscopic fields of view. The panels in the lower right show enlargements of representative areas from leaf areas inoculated with the ΔIVΔCEL mutant and the mutant expressing hopR1. The experiment was repeated six times with similar results. The scale bar is 100 µm.