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The Nucleocapsid Region of HIV-1 Gag Cooperates with the PTAP and LYPXnL Late Domains to Recruit the Cellular Machinery Necessary for Viral Budding

Figure 2

The N-terminal region of Bro1 inhibits the release of HIV-1.

(A) Schematic representation of constructs used in the experiment. Numbers indicate positions of amino acid residues. (B, C, D) 293T cells were transfected with either pNL4-3 plasmid alone or with increasing amounts of the indicated dominant-negative fragments of Alix. Pelleted virions and cell lysates were analyzed by SDS-PAGE and western blot using the indicated antibodies. (B) Over-expression of Bro1-V but not delBroV inhibited virus-release. (C) The Bro1-VF676D mutant retains inhibitory effect on HIV-1 release. (D) Broi, but not Bro1, inhibits HIV-1 release. (E) Relative release efficiency of HIV-1 virions upon over-expression of each of the four N-terminal fragments of Alix. Error bars indicate the standard deviation from three separate experiments.

Figure 2

doi: https://doi.org/10.1371/journal.ppat.1000339.g002