The Plasmodium falciparum STEVOR Multigene Family Mediates Antigenic Variation of the Infected Erythrocyte
Figure 3
Transcription and expression analyses of STEVOR.
(A) Immunoblotting of carbonate-insoluble pellet integral membrane proteins of schizont extracts of clones 5A, 5B, 3.2C, and 5.2A. A protein band of approximately 35 kDa was detected by anti-S1 serum in clones 5A and 3.2C (upper left panel) and by anti-S2 serum in clones 5A and 5.2A (upper right panel). Mouse anti-glycophorin C antibody recognizing a protein band of 25 kDa was used as loading control. Molecular weight comparison of detected STEVOR proteins by anti-S1 (lower left panel) and anti-S2 (lower right panel) is also shown. Secondary anti-rabbit (or mouse) IgG horseradish peroxydase-conjugated antibodies (Bio-Rad) and West Pico chemiluminescence (Pierce) were used to visualize the detected proteins in photographic film. Molecular marker (MM, Biorad) used for protein size comparison is shown. (B) Microarray analysis of the expression profile of transcribed stevor genes at mid-trophozoite stage (22–28 hours post invasion) of each clone. Level of transcription was determined in relation to a pool of RNA as described in Materials and Methods. Individual stevor genes with regulated transcription are shown by their accession number.