HCV Induces Oxidative and ER Stress, and Sensitizes Infected Cells to Apoptosis in SCID/Alb-uPA Mice
Figure 8
Immunohistochemical and confocal microscopic analysis of BAX expression.
BAX expression was examined by either immunohistochemistry (A–D) or confocal microscopy (E–F). Liver sections from PBS injected (A and E), replication deficient RNA injected (B) and HCV H77c infected (C, D and F) donor matched chimeric mice were stained using rabbit anti-BAX alone (A–D) and developed using the Vecastain ABC kit. Magnification ×100 (A–C). A magnification ×400 view is shown in D with red arrows indicating cells diffuse BAX, and black arrows indicating cells with punctate BAX. Isotype controls are shown in Figure S3 and are negative. For confocal microscopy, primary antibodies were rabbit anti-BAX with mouse anti-HCV, and the secondary antibodies were goat anti-rabbit alexa 488 (green), with goat anti-mouse poly-HRP, developed using tyramide-TMR (red). Nuclei were stained using DAPI (blue). Comparison of a field from an area of liver containing only mouse cells with an HCV infected human area is shown in Figure S3.